Cellulases from Thermophilic Fungi: Recent Insights and Biotechnological Potential

Table 1

Some properties of recombinant thermophilic fungal cellulases expressed in heterologous hosts.

Fungus	Gene	Family	Host	Optimal pH	pI	Optimal Temp (°C)	Thermal stability	Molecular mass (kDa)	Reference
Acremonium thermophilum	cel7a	7	Trichoderma reesei	5.5	4.67	60	NR	53.7	[15]
Chaetomium thermophilum	cel7a	7	Trichoderma reesei	4	5.05	65	NR	54.6	[15]
Chaetomium thermophilum	cbh3	7	Pichia pastoris	4	5.15	60	𝑇1/2: 45 min at 70°C	50.0	[16]
Humicola grisea	egl2	5	Aspergillus oryzae	5	6.92	75	80% residual activity for 10 min at 75°C	42.6	[17]
Humicola grisea	egl3	45	Aspergillus oryzae	5	5.78	60	75% residual activity for 10 min at 80°C	32.2	[17]
Humicola grisea	egl4	45	Aspergillus oryzae	6	6.44	75	75% residual activity for 10 min at 80°C	24.2	[18]
Humicola grisea var thermoidea	eg1	7	Aspergillus oryzae	5	6.43	55–60	Stable for 10 min at 60°C	47.9	[19]
Humicola grisea var thermoidea	cbh1	7	Aspergillus oryzae	5	4.73	60	Stable for 10 min at 55°C	55.7	[19]
Humicola insolens	avi2	6	Humicola insolens	NR	5.65	NR	NR	51.3	[20]
Humicola insolens	cbhII	6	Saccharomyces cerevisiae	9	NR	57	𝑇1/2: 95 min at 63°C	NR	[21, 22]
Melanocarpus albomyces	cel7b	7	Trichoderma reesei	6–8	4.23	NR	NR	50.0	[23]
Melanocarpus albomyces	cel7a	7	Trichoderma reesei	6–8	4.15	NR	NR	44.8	[23]
Melanocarpus albomyces	cel45a	45	Trichoderma reesei	6–8	5.22	NR	NR	25.0	[23]
Talaromyces emersonii	cel3a	3	Trichoderma reesei	4.02	3.6	71.5	𝑇1/2: 62 min at 65°C	90.6	[24]
Talaromyces emersonii	cel7	7	E. coli	5	4.0	68	𝑇1/2: 68 min at 80°C	48.7	[7]
Talaromyces emersonii	cel7A	7	Saccharomyces cerevisiae	4-5		65	𝑇1/2: 30 min at 70°C	46.8	[25]
Thermoascus aurantiacus	cbh1	7	Saccharomyces cerevisiae	6	4.37	65	80% residual activity for 60 min at 65°C	48.7	[26]
Thermoascus aurantiacus	eg1	5	Saccharomyces cerevisiae	6	4.36	70	stable for 60 min at 70°C	37.0	[27]
Thermoascus aurantiacus	bgl1	3	Pichia pastoris	5	4.61	70	70% residual activity for 60 min at 60°C	93.5	[28]
Thermoascus aurantiacus	cel7a	7	Trichoderma reesei	5	4.44	65	NR	46.9	[15]