Effect of Culture Conditions on the Production of an Extracellular Protease by Bacillus sp. Isolated from Soil Sample of Lavizan Jungle Park

Table 4

Effect of agitation rate on growth and protease activity by Bacillus sp. strain CR-179 cultivated in a liquid medium containing starch (1%) and corn steep liquor (0.4%) in shake flasks during 24 h at 45°C.

Agitation rate (rpm)	Culture density (OD 600 nm)	Maximum enzyme activity (U/mL)
110	1.327	301.688
130	1.388	320.389
150	1.418	345.844
180	1.330	307.142