TY - JOUR A2 - Cabral, J. M. S. AU - de Castro, Aline Machado AU - Ferreira, Marcela Costa AU - da Cruz, Juliana Cunha AU - Pedro, Kelly Cristina Nascimento Rodrigues AU - Carvalho, Daniele Fernandes AU - Leite, Selma Gomes Ferreira AU - Pereira, Nei PY - 2010 DA - 2010/09/26 TI - High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct SP - 854526 VL - 2010 AB - The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol. In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation. This fungal strain produced high contents of endoglucanase activity (6,358 U·L1) after 72 hours of process, and further relevant β-glucosidase and FPase activities (742 and 445 U·L1, resp.). The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50C, accessory xylanase activity, and absence of proteolytic activity towards azocasein. This strain showed, therefore, potential for the production of complete cellulolytic complexes aiming at the saccharification of lignocellulosic materials. SN - 2090-0406 UR - https://doi.org/10.4061/2010/854526 DO - 10.4061/2010/854526 JF - Enzyme Research PB - SAGE-Hindawi Access to Research KW - ER -