TY - JOUR
A2 - Cabral, J. M. S.
AU - de Castro, Aline Machado
AU - Ferreira, Marcela Costa
AU - da Cruz, Juliana Cunha
AU - Pedro, Kelly Cristina Nascimento Rodrigues
AU - Carvalho, Daniele Fernandes
AU - Leite, Selma Gomes Ferreira
AU - Pereira, Nei
PY - 2010
DA - 2010/09/26
TI - High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct
SP - 854526
VL - 2010
AB - The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol. In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation. This fungal strain produced high contents of endoglucanase activity (6,358 U·L−1) after 72 hours of process, and further relevant β-glucosidase and FPase activities (742 and 445 U·L−1, resp.). The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50∘C, accessory xylanase activity, and absence of proteolytic activity towards azocasein. This strain showed, therefore, potential for the production of complete cellulolytic complexes aiming at the saccharification of lignocellulosic materials.
SN - 2090-0406
UR - https://doi.org/10.4061/2010/854526
DO - 10.4061/2010/854526
JF - Enzyme Research
PB - SAGE-Hindawi Access to Research
KW -
ER -