Green Tea Leaves and Rosemary Extracts Selectively Induce Cell Death in Triple-Negative Breast Cancer Cells and Cancer Stem Cells and Enhance the Efficacy of Common Chemotherapeutics

Figure 7

RE induces ROS production in triple-negative breast cancer. (a) MDA-MB-231 breast cancer cells were treated with H2DCFDA following treatments with Taxol and cisplatin individually and in combination with STE and RE. Results were obtained by using image-based cytometry to assess the percentage of cells positive with DCF, which indicates the generation of ROS. Hydrogen peroxide served as a positive control. Treatment groups were compared with the negative control DMSO. Values are expressed as a mean ± SD from three independent experiments. Statistical calculations were performed by using a two-way ANOVA multiple comparison test. vs control, vs control, and vs control. (b) Fluorescent microscopy of triple-negative breast cancer cells stained with H2DCFDA (green) and counterstained with Hoechst (blue) at 200x magnification. Cells fluorescing green indicate the generation of ROS. Images are representative of three independent experiments.

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