Green Tea Leaves and Rosemary Extracts Selectively Induce Cell Death in Triple-Negative Breast Cancer Cells and Cancer Stem Cells and Enhance the Efficacy of Common Chemotherapeutics

Figure 3

Synthite tea and rosemary extracts positively interacted with each other and with chemotherapeutics in triple-negative breast cancer. MDA-MB-231 cells were treated with varying combination therapies: (a) 0.10 mg/mL of Synthite tea and 0.025 mg/mL of rosemary, (b) 0.50 µM of cisplatin on its own and in combination with both extracts, and (c) 0.01 µM of Taxol on its own and in combination with both extracts. The graphed results were obtained by using image-based cytometry to assess the percentage of cells positive with fluorescence associated with Annexin V (green), PI (red), and both (yellow) or negative for both Annexin V and PI (blue). Values are expressed as a mean ± SD from three independent experiments. All treatment groups are compared with a negative control of DMSO. vs control, vs control, and vs control.

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