Green Tea Leaves and Rosemary Extracts Selectively Induce Cell Death in Triple-Negative Breast Cancer Cells and Cancer Stem Cells and Enhance the Efficacy of Common Chemotherapeutics

Figure 10

Immunofluorescence staining of stem cells with CD44 stem cell surface markers in cells treated with STE, RE, cisplatin, Taxol, and herb-drug combinations at 48 hours. Bars represent the average percentage of cells expressing CD44 for each group (n = 3) in MDA-MB-231 at 48 hours following treatment. Error bars represent the positive and negative SD of each group. , , , and compared to the control. ^‡ compared to cisplatin alone. ^† compared to Taxol alone. Micrographs are overlays of fluorescence and brightfield images at 200x magnification. Scale bar is 100 μm. Each micrograph is representative of the three independent experiments. Cells were stained with Hoechst 33342 (blue) and CD44 (green). (a) Immunofluorescence staining of CSCs with CD44 in cells treated with STE, RE, and a combination of STE and RE. (b) Immunofluorescence staining of stem cells with CD44 stem cell surface markers in cells treated with cisplatin and herb-cisplatin combinations. (c) Immunofluorescence staining of stem cells with CD44 stem cell surface markers in cells treated with Taxol and herb-Taxol combinations.

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