Evaluation of the Protoscolicidal Activity of Humulus lupulus Methanolic Extracts on Echinococcus granulosus Sensu Stricto

Figure 2

Light microscopy of E. granulosus s.s. protoscoleces incubated in vitro with the different Humulus lupulus varieties. Control protoscoleces remained unaltered throughout the experiment (a). Protoscoleces treated with Mapuche during 3 days showed soma contraction (arrow), rostellar disorganization (arrow head) and presence of blebs in the tegument (b), and after 9 days of treatment an important reduction in vitality was observed (c). The treatment with the variety Victoria caused the first morphological alterations at day 6 (d), which were more pronounced at day 12 (e). The varieties Bullion (f), Cascade (g), and Traful (h) caused a slower effect. Asterisks point dead protoscoleces.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)