[Retracted] Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice

Figure 1

GDF7 alleviates LPS-induced ALI and pulmonary dysfunction in mice. (a) Mice were intratracheally injected with LPS (5 mg/kg in 50 μL saline) to generate sepsis-induced ALI, and serum GDF7 levels were detected using an ELISA kit after 12 h (n = 6). (b) The levels of Gdf7 mRNA in lung tissues with or without LPS instillation were detected using quantitative real-time PCR (n = 6). (c) The levels of GDF7 protein in lung tissues with or without LPS instillation were detected using western blot (n = 6). (d-e) Mice were subcutaneously injected with rmGDF7 (25 μg per mouse), and serum and lung GDF7 levels in mice were detected using an ELISA kit after 24 h (n = 6). (f) Mice were subcutaneously injected with rmGDF7 (25 μg per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μL saline) to generate sepsis-induced ALI. After 12 h, fresh lungs were harvested for the analysis of LDH activity using a commercial kit (n = 6). (g) Lung wet to dry ratio (n = 6). (h) Total proteins in BALF (n = 6). (i) Arterial blood gas analysis results (n = 6). (j) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance (n = 6). (k) Mice were subcutaneously injected with rmGDF7 (25 μg per mouse), and 24 h later, ALI mice were intratracheally injected with a lethal dose of LPS (25 mg/kg). The survival rate was monitored every 12 h post-LPS treatment (n = 20). All data were presented as mean ± SD, and  < 0.05 was regarded to be statistically significant.

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