TLC-Based Metabolite Profiling and Bioactivity-Based Scientific Validation for Use of Water Extracts in AYUSH Formulations

Table 3

DPPH free radical scavenging and reducing power capacity of different extracts of plant materials.

S. no.	Plant sample	DPPH scavenging activity (IC50 (μg/mL))			Reducing power capacity (μg/mL); Mean ± SD
		WE	EE	HEE	WE	EE	HEE
1	Phyllanthus emblica	97.26 ± 1.14	124.89 ± 3.45	115.45 ± 2.45	100 ± 10	160 ± 20	120 ± 10^ns
2	Piper nigrum	112.18 ± 2.11	222.34 ± 2.36	210.89 ± 0.45	140 ± 20	240 ± 10	220 ± 10
3	Withania somnifera	85.96 ± 3.42	115.65 ± 3.63	91.26 ± 1.11^ns	100 ± 10	140 ± 20	120 ± 10^ns
4	Tinospora cordifolia	105.65 ± 3.33	185.56 ± 2.56	177.23 ± 2.31	120 ± 10	200 ± 10	180 ± 20
5	Curcuma longa	199.15 ± 0.42	285.47 ± 4.56	205.66 ± 1.75^ns	200 ± 30	300 ± 10	220 ± 20^ns
6	Ocimum sanctum	201.56 ± 1.11	245.25 ± 3.21	223.45 ± 3.33	240 ± 20	280 ± 20^ns	220 ± 10^ns
7	Azadirachta indica	156.83 ± 0.88	171.22 ± 2.56	162.32 ± 2.11	160 ± 10	200 ± 20^ns	180 ± 30^ns
8	Achillea millefolium	185.65 ± 1.23	145.56 ± 2.22	152.23 ± 2.56	160 ± 20	180 ± 20^ns	200 ± 20^ns
9	Ascorbic acid	55.37 ± 1.25	80 ± 10

Data are expressed as mean ± SD (n = 3). One-way ANOVA followed by Tukey’s multiple comparisons test. Compared to WE (water extract): , , ; ns .WE = water extract; EE = ethanolic extract; HEE = hydroethanolic extract.