TLC-Based Metabolite Profiling and Bioactivity-Based Scientific Validation for Use of Water Extracts in AYUSH Formulations

Table 2

Total phenolic and flavonoid contents of different extracts of plant materials.

S. no.	Plant sample	TPC (%); Mean ± SD			TFC (%); Mean ± SD
		WE	EE	HEE	WE	EE	HEE
1	Phyllanthus emblica	31.25 ± 0.94	27.55 ± 0.54	42.35 ± 1.70	16.24 ± 1.91	10.21 ± 1.09	20.15 ± 1.22
2	Piper nigrum	12.31 ± 1.21	10.54 ± 0.90^ns	20.38 ± 2.16	06.44 ± 0.79	05.24 ± 0.84^ns	11.56 ± 1.49
3	Withania somnifera	26.25 ± 0.45	22.75 ± 1.02^ns	38.42 ± 1.54	11.24 ± 1.15	09.92 ± 0.99^ns	19.58 ± 1.58
4	Tinospora cordifolia	14.11 ± 1.10	11.85 ± 0.94^ns	19.30 ± 1.24	06.55 ± 0.62	04.18 ± 0.83^ns	14.85 ± 1.88
5	Curcuma longa	09.91 ± 0.17	8.12 ± 0.90^ns	12.78 ± 1.10	05.87 ± 1.14	04.09 ± 0.80^ns	13.77 ± 1.30
6	Ocimum sanctum	17.21 ± 1.26	13.89 ± 0.95	26.48 ± 1.55	10.45 ± 1.67	07.35 ± 0.77^ns	18.08 ± 2.62
7	Azadirachta indica	25.54 ± 1.00	19.57 ± 0.68	34.46 ± 2.99	14.45 ± 1.54	12.34 ± 1.91^ns	17.64 ± 1.15^ns
8	Achillea millefolium	14.21 ± 1.05	13.54 ± 1.15^ns	19.51 ± 2.18	06.87 ± 0.86	04.28 ± 0.80	10.24 ± 0.81

Data are expressed as mean ± SD (n = 3). One-way ANOVA followed by Tukey’s multiple comparison tests. Compared to WE (water extract): , , ; ns . TPC = total phenolic content; TFC = total flavonoid content; WE = water extract; EE = ethanolic extract; HEE = hydroethanolic extract.