Cordyceps cicadae Prevents Renal Tubular Epithelial Cell Apoptosis by Regulating the SIRT1/p53 Pathway in Hypertensive Renal Injury

Figure 7

Effects of medicated serum containing Cordyceps cicadae (CSM) on SIRT1 and acetylated p53 in tubular epithelial cells (TECs) treated with angiotensin II (AngII). (a) Western blot analysis of SIRT1 and acetylated p53 in the TECs exposed to 10⁻⁷ mM AngII or vehicle treated with CSM or control serum. Densitometry was performed for quantification, and ratios of SIRT1 and acetylated-p53 to tubulin were expressed as a fold of the control. (b) Immunofluorescent staining for SIRT1 (red) and acetylated p53 (green) in TECs as described in (a). Scale bar: 20 μm. Average fluorescence intensity was performed for quantification. CSM upregulated SIRT1 expression in the AngII-treated TECs and downregulated p53 acetylation. and , as compared with the control TECs. as compared with the TECs exposed to AngII.

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