Cordyceps cicadae Prevents Renal Tubular Epithelial Cell Apoptosis by Regulating the SIRT1/p53 Pathway in Hypertensive Renal Injury

Figure 6

Effects of Cordyceps cicadae (C. cicadae) and resveratrol (RES) on the expression levels of SIRT1 and acetylated-p53 in tubular epithelial cells (TECs) of spontaneously hypertensive rats (SHRs). (a) Immunohistochemical staining for SIRT1 and acetylated p53 in the renal tubules of the rats in each group. Scale bar: 100 μm. The optical intensity of the SIRT1 and acetylated p53 was measured. (b) Capillary electrophoresis immunoquantification of SIRT1 in the renal cortex of the rats in each group. Densitometry was performed for quantification, and the ratio of SIRT1 to GAPDH was expressed as a fold of the control. C. cicadae: SHRs treated with 4 g/kg/d C. cicadae; RES : SHRs treated with 40 mg/kg/d resveratrol. C. cicadae increased SIRT1 expression and decreased p53 acetylation in the renal tubules of the SHRs, similar to the effects of resveratrol. and , as compared with the WKY. , , and , as compared with the SHR.

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