Cordyceps cicadae Prevents Renal Tubular Epithelial Cell Apoptosis by Regulating the SIRT1/p53 Pathway in Hypertensive Renal Injury

Figure 3

Effects of Cordyceps cicadae (C. cicadae) and resveratrol (RES) on tubular epithelial cell (TEC) injury induced by angiotensin II (AngII). (a) The level of urinary kim-1 was measured with enzyme-linked immunosorbent assay (ELISA) in the rats in all the groups. C. cicadae: spontaneously hypertensive rats (SHRs) treated with 4 g/kg/d C. cicadae; RES: SHRs treated with 40 mg/kg/d resveratrol. (b, c) Levels of kim-1 and NGAL were measured with ELISA in culture supernatants of TECs treated with 10% CSM and/or 10 μM EX527 in the presence or absence of 10⁻⁷ mM AngII. (d–g) Levels of kim-1 and NGAL were measured with ELISA in culture supernatants of TECs. The CSM and RES treatments reduced the secretion of kim-1 and NGAL in the TECs after exposure to AngII, and EX527 reversed the beneficial effect of CSM. EP: TECs treated with 12.5 μg/ml ergosterol peroxide. RES: TECs treated with 25 μM resveratrol. and , as compared with the WKY or control TECs. and , as compared with SHRs or TECs exposed to AngII. , as compared with TECs exposed to AngII plus CSM.

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