Cordyceps cicadae Prevents Renal Tubular Epithelial Cell Apoptosis by Regulating the SIRT1/p53 Pathway in Hypertensive Renal Injury

Figure 2

Effects of Cordyceps cicadae (C. cicadae) and resveratrol (RES) on tubulointerstitial fibrosis and fibrosis-relative protein induced by hypertension. (a) Hematoxylin-eosin (H&E) staining: no distinct pathological differences amongst the groups were observed. (b, d) Masson staining: SHR group showed severe collagen deposition in renal interstitium. C. cicadae and RES significantly reduced the deposition of collagen. (c) Immunohistochemical staining of α-smooth muscle actin (α-SMA) in each group. (e) Average optical intensity of α-SMA was measured. The expression of α-SMA significantly increased in spontaneously hypertensive rats (SHRs), which was reduced after treatment with C. cicadae and RES. Scale bar: 100 μm. C. cicadae: SHRs treated with 4 g/kg/d C. cicadae; RES : SHRs treated with 40 mg/kg/d resveratrol. , as compared with the WKY, , , and , as compared with SHR.

(a)

(b)

(c)

(d)

(e)