Antidiarrheal Activity of Hydromethanolic Root Extract and Solvent Fractions of Clutia abyssinica Jaub. & Spach. (Euphorbiaceae) in Mice

Table 1

Antidiarrheal effects of C. abyssinica extract and solvent fractions.

Treatments given		Onset of wet stool appearance (min)	Number of wet stools	Number of total stools	% reduction of wet stools
Negative control		72.00 ± 7.86	10.83 ± 0.79	14.33 ± 0.99	—
Extract (mg/kg)	100	131.67 ± 12.65^e	7.33 ± 1.26^e	10.50 ± 1.46^d^e	32.32
	200	147.17 ± 18.05^a	5.00 ± 0.86^a	7.50 ± 1.26^a	53.83
	400	158.00 ± 14.64^a	3.50 ± 0.22^a	5.50 ± 0.43^a^b	67.68
Solvent fractions (mg/kg)	CF100	88.50 ± 7.20^d^e	7.83 ± 0.70^d^e	10.50 ± 0.96^d^e	27.70
	CF200	106.17 ± 6.72^e	6.50 ± 0.56^a^e	9.67 ± 0.76^a^e	39.98
	CF400	180.00 ± 15.30^a	4.00 ± 0.45^a	5.83 ± 0.60^a^b	63.07
	n-BF100	83.33 ± 12.46^d^e	7.17 ± 1.20^e	9.67 ± 0.88^a^e	33.80
	n-BF200	87.50 ± 11.70^d^e	6.33 ± 0.92^a	8.67 ± 1.02^a	41.55
	n-BF400	119.33 ± 23.02^e	4.17 ± 0.54^a	7.17 ± 0.70^a	61.50
	AF100	91.00 ± 11.28^e	5.67 ± 1.36^a	8.17 ± 1.20^a	47.65
	AF200	102.00 ± 13.02^e	4.50 ± 0.56^a	7.00 ± 0.37^a	58.45
	AF400	120.50 ± 17.87^e	3.67 ± 0.76^a	6.00 ± 0.68^a	66.11
Positive control		199.00 ± 8.77^a^b	2.33 ± 0.62^a^b	4.67 ± 0.72^a^b	80.95

Values are expressed as mean ± SEM (); analysis was performed using one-way ANOVA followed by Tukey’s post hoc test; ^acompared to negative control, ^bcompared to 100 mg/kg crude extract, ^ccompared to 200 mg/kg crude extract, ^dcompared to 400 mg/kg crude extract, ^ecompared to positive control. ; ; . CF = Chloroform fraction; n-BF = n-butanol fraction; AF, aqueous fraction.