Optimization of Extraction Conditions of Phytochemical Compounds and Anti-Gout Activity of Euphorbia hirta L. (Ara Tanah) Using Response Surface Methodology and Liquid Chromatography-Mass Spectrometry (LC-MS) Analysis

Table 4

Tentative identification of chemical constituents in the E. hirta water extract.

No	Tentative assignment	RT (min)	Molecular weight	Precursor type	Precursor m/z	Molecular formula	MS/MS fragment ions	References
1	Neochlorogenic acid	2.2	354.09	[M − H]^−	353	C16 H18 O9	191, 179	Massbank: PM013904
2	Caffeic acid	3.46	180.04	[M − H]^−	179.0341	C9 H8 O4	135, 179, 134	Massbank: FiehnHILIC001102
3	Syringic acid	5.48	198.052	[M − H]^−	197.045	C9 H10 O5	182, 166, 197	Massbank: FiehnHILIC001522
4	Ellagic acid	6.47	302.006	[M − H]^−	300.999	C14 H6 O8	303, 300	Massbank: FiehnHILIC001170
5	Quercetin-3β-D-glucoside	6.89	464.09	[M − H]^−	463.0879	C21 H20 O12	300, 301, 271	Pubchem (CID5280804)
6	Astragalin	8.53	448.1	[M − H]^−	447.094	C21 H20 O11	447, 285, 284	Massbank: CCMSLIB00000845312
7	Afzelin	9.34	432.1	[M + FA − H]^−	477.104	C21 H20 O10	285, 431, 284	Massbank: CCMSLIB00000845703
8	Quercetin	11.25	302.04	[M − H]^−	301.0359	C15 H10 O7	107, 121, 151, 178, 301	Massbank: FiehnHILIC002955
9	Corchorifatty acid F	15.06	328.224	[M − H]^−	346.081	C18 H32 O5	235, 311, 219	Pubchem (CID44559173)
10	2-Undecanone 2,4-dinitrophenylhydrazone	15.08	350.4	[M + H]^+	351.4	C17 H26 N4 O4	351, 352	Pubchem (CID 5717665)
11	Demexiptiline	27.16	278.3	[M + H]^+	279.3	C18 H18 N2 O	204, 203, 149	Massbank: WA002119