Electroacupuncture Ameliorates Cerebral I/R-Induced Inflammation through DOR-BDNF/TrkB Pathway

Figure 1

DOR activation increases cell viability in OGD/R-exposed PC12 cells. The cellular morphological changes, cell viability, and LDH level were evaluated after exposure to 6 h OGD followed by 24 h reperfusion (OGD/R). The DOR agonist or antagonist treatment is 30 min before OGD. In the coadministration group, antagonist NTI was added to the cell culture medium 30 min before the agonist Tan67 incubation. (a) Representative images of PC12 cells in the sham group, OGD/R group, agonist group, antagonist group, and coadministration group. Cell viability was measured by (b) CCK-8 assay and (c) LDH release. Activation of DOR attenuated the PC12 cells injury, whereas it was totally abolished by preincubation of DOR antagonist. Scale bar = 50 μm. N = 6 in each group. and vs. control group. and vs. OGD/R group.

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