Evidence-Based Complementary and Alternative Medicine / 2019 / Article / Fig 6

Research Article

Anti‐Aging Effects of Schisandrae chinensis Fructus Extract: Improvement of Insulin Sensitivity and Muscle Function in Aged Mice

Figure 6

Supplementation with SFe decreased mRNA and protein expression of muscle degradation factors in aged mice. (a) The mRNA expression of atrogin-1, MuRF-1, and myostatin in TA muscle was analyzed by qRT-PCR, with 18S rRNA used as an internal control. The values are expressed as the relative fold change compared with the 16M CON group (n = 5–7 per group). (b) Atrogin-1, MuRF-1, and MSTN protein expression in TA muscle was analyzed by using western blotting, with GAPDH expression used as an internal control. Representative images of the blots are shown in the left panel; quantification of the blots was computed using ImageJ software and is shown in the right panel. The quantitation values are expressed as the relative fold change compared with the 16M CON group. The data are presented as the mean ± standard deviation (SD) (n = 10 per group). .
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