Evidence-Based Complementary and Alternative Medicine / 2019 / Article / Fig 5

Research Article

Anti‐Aging Effects of Schisandrae chinensis Fructus Extract: Improvement of Insulin Sensitivity and Muscle Function in Aged Mice

Figure 5

Supplementation with SFe increased muscle protein synthesis in aged mice. (a) The mRNA expression of MyHC-1, -2A, -2X, -2B, MRF4, and MyoG in TA muscle was analyzed by using qRT-PCR, with 18S rRNA used as an internal control. The values are expressed as the relative fold change as compared with the 16M CON group. (b) MyoG, MyHC, Myf5, and MyoD protein expression in TA muscle were analyzed by western blotting, with GAPDH expression analyzed as an internal control. Representative images of the blots are shown in the left panel; quantification of the blots was computed using ImageJ software and is shown in the right panel. The quantitation values are expressed as the relative fold change compared with the 16M CON group. The data are presented as the mean ± standard deviation (SD) (MyoG and MyHC, n = 10 per group; Myf5 and MyoD, n = 4 per group). ; ; and .
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