Multiresponse Optimization of Pomegranate Peel Extraction by Statistical versus Artificial Intelligence: Predictive Approach for Foodborne Bacterial Pathogen Inactivation

Table 5

Analyses of covariance for all dependent variables and effect of S. aureus ATCC 6538 and S. enterica ATCC 14028 activities.

Parameter	Staphylococcus aureus ATCC 6538							Salmonella enterica subsp. enterica serovar typhimurium ATCC 14028
	Estimate	SE	df	t	Sig.	LB	UB	Estimate	SE	df	t	Sig.	LB	UB
Intercept	9.44	2.432	60	3.88	0.000	4.573	14.306	6320	3203.043	0	1.973	1.000	−79955.35	92595.35
Hour 0	−5.44	3.44	60	−1.581	0.119	−12.321	1.441	−6316	4529.787	0	−1.394	1.000	−128327.77	115695.77
Hour 1	−5.089	3.44	60	−1.479	0.144	−11.97	1.792	−2070	4529.787	0	−0.457	1.000	−124081.77	119941.77
Hour 2	−4.64	3.44	60	−1.349	0.182	−11.521	2.241	−1820	4529.787	0	−0.402	1.000	−123831.77	120191.77
Hour 3	−4.42	3.44	60	−1.285	0.204	−11.301	2.461	−1420	4529.787	0	−0.313	1.000	−123431.77	120591.77
Hour 4	−3.859	3.44	60	−1.122	0.266	−10.74	3.022	−844	4529.787	0	−0.186	1.000	−122855.77	121167.77
Hour 5	−2.623	3.44	60	−0.763	0.449	−9.504	4.258	−89	4529.787	0	−0.020	1.000	−122100.77	121922.77
Hour 6	−2.079	3.44	60	−0.605	0.548	−8.961	4.801	166	4529.787	0	0.037	1.000	−121845.77	122177.77
Hour 7	−1.248	3.44	60	−0.363	0.718	−8.129	5.633	352	4529.787	0	0.078	1.000	−121659.77	122363.77
Hour 8	−1.018	3.44	60	−0.296	0.768	−7.9	5.863	348	4529.787	0	0.077	1.000	−121663.77	122359.77
Hour 9	−0.672	3.44	60	−0.196	0.846	−7.554	6.208	422.8	4529.787	0	0.093	1.000	−121588.97	122434.57
Hour 10	−0.51	3.44	60	−0.148	0.882	−7.392	6.37	500	4529.787	0	0.110	1.000	−121511.77	122511.77
Hour 11	−0.712	3.44	60	−0.207	0.837	−7.593	6.169	491	4529.787	0	0.108	1.000	−121520.77	122502.77
Hour 12	−0.665	3.44	60	−0.193	0.847	−7.546	6.216	511	4529.787	0	0.113	1.000	−121500.77	122522.77
Hour 14	−0.181	3.44	60	−0.053	0.958	−7.062	6.7	304	4529.787	0	0.067	1.000	−121707.77	122315.77
Hour 16	−0.029	3.44	60	−0.008	0.993	−6.91	6.852	11	4529.787	0	0.002	1.000	−122000.77	122022.77
Hour 18	0.096	3.44	60	0.028	0.978	−6.785	6.977	−44	4529.787	0	−0.010	1.000	−122055.77	121967.77
Hour 20	0.205	3.44	60	0.060	0.953	−6.676	7.086	−70	4529.787	0	−0.015	1.000	−122081.77	121941.77
Hour 22	0.183	3.44	60	0.053	0.958	−6.698	7.064	−190	4529.787	0	−0.042	1.000	−122201.77	121821.77
Hour 24	0.087	3.44	60	0.025	0.980	−6.794	6.968	−120	4529.787	0	−0.026	1.000	−122131.77	121891.77
Hour 26	0^a	0	60	—	—	—		0^a	0	—	—	—	—	—
Trial	−2.11	0.733	60	−2.877	0.006	−3.577	−0.642	−1330	422.878	60	−3.145	0.003	−2175.882	−484.117
Hour 0 × trial	2.11	1.037	60	2.034	0.016	0.035	4.184	1330	598.04	60	2.224	0.009	133.741	2526.258
Hour 1 × trial	2.11	1.037	60	2.034	0.022	0.035	4.184	1330	598.04	60	2.224	0.011	133.741	2526.25
Hour 2 × trial	2.11	1.037	60	2.034	0.029	0.035	4.184	1330	598.04	60	2.224	0.015	133.741	2526.258
Hour 3 × trial	2.11	1.037	60	2.034	0.034	0.035	4.184	1330	598.04	60	2.224	0.030	133.741	2526.258
Hour 4 × trial	1.691	1.037	60	1.631	0.044	−0.3835	3.766	1054	598.04	60	1.762	0.039	−142.258	2250.258
Hour 5 × trial	1.326	1.037	60	1.279	0.046	−0.7486	3.401	755	598.04	60	1.262	0.044	−441.258	1951.258
Hour 6 × trial	1.13	1.037	60	1.090	0.206	−0.9447	3.205	586	598.04	60	0.980	0..049	−610.258	1782.258
Hour 7 × trial	0.775	1.037	60	0.748	0.208	−1.299	2.85	428	598.04	60	0.716	0.077	−768.258	1624.258
Hour 8 × trial	0.574	1.037	60	0.554	0.481	−1.5	2.649	352	598.04	60	0.589	0.258	−844.258	1548.258
Hour 9 × trial	0.476	1.037	60	0.459	0.548	−1.598	2.551	256.6	598.04	60	0.429	0.369	−939.658	1452.858
Hour 10 × trial	0.376	1.037	60	0.363	0.718	−1.698	2.451	144	598.04	60	0.241	0.511	−1052.258	1340.258
Hour 11 × trial	0.358	1.037	60	0.345	0.731	−1.716	2.432	115	598.04	60	0.192	0.748	−1081.258	1311.258
Hour 12 × trial	0.275	1.037	60	0.265	0.792	−1.799	2.349	29	598.04	60	0.048	0.861	−1167.258	1225.258
Hour 14 × trial	0.111	1.037	60	0.107	0.915	−1.96	2.185	14	598.04	60	0.023	0.981	−1182.258	1210.2584
Hour 16 × trial	0.045	1.037	60	0.043	0.966	−2.029	2.119	51	598.04	60	0.085	0.932	−1145.258	1247.258
Hour 18 × trial	0.006	1.037	60	0.006	0.995	−2.068	2.08	40	598.04	60	0.067	0.947	−1156.258	1236.258
Hour 20 × trial	−0.029	1.037	60	−0.028	0.978	−2.103	2.045	30	598.04	60	0.050	0.960	−1166.258	1226.258
Hour 22 × trial	−0.029	1.037	60	−0.028	0.978	−2.103	2.045	50	598.04	60	0.084	0.934	−1146.258	1246.258
Hour 24 × trial	−0.015	1.037	60	−0.015	0.988	0–2.09	2.059	30	598.04	60	0.050	0.960	−1166.258	1226.258
Hour 26 × trial	0^a	0	—	—	—	—	—	0^a	0	—	—	—	—	—

SE : Standard Error; df: Degrees of freedom; t: the Student t-statistic; Sig.: the value (associated with the correlation); LB : Lower bound. UB : Upper bound; Trial 1: negative control; Trial 2 : 1 × MIC. Trial 3 : 2 × MIC; Trial 4 : 4 × MIC and Trial 5 : 8 × MIC; ; ; , ^aThis parameter is set to zero because it is redundant;^b Dependent variable: CFU.