Neuroprotective Effect and Molecular Mechanism of [6]-Gingerol against Scopolamine-Induced Amnesia in C57BL/6 Mice

Figure 6

Effect of GIN on the SCO-reduced protein expression of BDNF in the cortex. Mice were pretreated with GIN for 3 days before the start of the test and then continuously administered during the behavior tests. The protein expression ratios of BDNF/actin (a) and p-Akt/Akt (c) were monitored via western blot analysis. Data were presented as mean ± SEM (). Significant difference between the groups: , vehicle-treated control versus SCO group; , SCO alone group versus GIN-treated group in combination with SCO. (b) Effect of GIN on the activation of CREB via phosphorylation in the cortex was examined via immunohistochemistry by staining with anti-p-CREB antibody (right panel). (A) sham control, (B) SCO (1 mg/kg), (C) SCO (1 mg/kg) + GIN (10 mg/kg), (D) SCO (1 mg/kg) + GIN (25 mg/kg). The protein levels of p-CREB and CREB were also verified by western blot analysis (left panel).

(a)

(b)

(c)