Potent Phosphodiesterase Inhibition and Nitric Oxide Release Stimulation of Anti-Impotence Thai Medicinal Plants from “MANOSROI III” Database

Table 2

Phosphodiesterases (PDEs) inhibition and nitric oxide (NO) release stimulation activities of the fifteen selected plant extracts.

Number	Samples	Inhibition of PDEs activity				Stimulation of NO release
		%		IC50 (mg/mL)		%		SC50 (mg/mL)
		Mean ± S.D.	Folds of sildenafil	Mean ± SD	Folds of sildenafil	Mean ± SD	Folds of acetylcholine	Mean ± SD	Folds of acetylcholine
1	EDP1-001(1)			0.0026±0.0025	2.23
2	EDP1-001(2)			—	—			—	—
3	EDP1-001(3)			—	—			—	—
4	EDP1-002(1)			—	—			—	—
5	EDP1-002(2)			—	—			—	—
6	EDP1-003			—	—
7	EDP1-004			—	—			—	—
8	EDP2-001(1)			—	—			—	—
9	EDP2-001(2)			—	—			—	—
10	EDP2-001(3)			—	—			—	—
11	EDP2-002(1)			—	—			—	—
12	EDP2-002(2)			—	—			—	—
13	EDP2-003(1)			—	—			—	—
14	EDP2-003(2)			—	—			—	—
15	EDP2-003(3)			—	—			—	—
16	Sildenafil				1	—	—	—	—
17	Acetylcholine	—	—	—	—		1		1
18	L-NAME	—	—	—	—

Inhibition of PDEs activity: inhibition percentages were determined at the concentrations of the extracts and sildenafil at 200 and 0.45 µg/mL, respectively. IC₅₀ was the concentrations of samples giving 50% inhibition of the PDEs activity. The minus value indicated the stimulation of PDEs. Stimulation of NO release: stimulation percentages of the extracts, acetylcholine, and L-NAME were tested at the concentration of 1 mg/mL. SC₅₀ was the concentrations of samples giving 50% stimulation of the NO production activity. L-NAME was -nitro-L-arginine methyl ester hydrochloride (NO release inhibitor). The minus value indicated the inhibition of NO production.