Pharmacological Effect of Caulophyllum robustum on Collagen-Induced Arthritis and Regulation of Nitric Oxide, NF-κB, and Proinflammatory Cytokines In Vivo and In Vitro

Figure 2

Effects of CRME on cytokines in RAW264.7 cells. The cells were pretreated with different concentrations (0.250, 0.125, or 0.063 mg/mL) of CRME for 1 h and then continuously incubated with 1 μg/mL LPS for 24 h. Inhibition of NO in RAW264.7 cell supernatant by Griess method. (a) Inhibition of NO in RAW264.7 cells supernatant by Griess method. (b) Real-time PCR showing the TNF-α mRNA expression in RAW264.7 cells. (c) Real-time PCR showing the IL-1β mRNA expression in RAW264.7 cells. (d) Real-time PCR showing the IL-6 mRNA expression in RAW264.7 cells. All values are expressed as the mean ± SEM from three independent experiments. Data were analyzed by two-way ANOVA, Student-Newman-Keuls test as post hoc test (, in comparison with control; , in comparison with LPS).

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