A Molecular Basis for the Inhibition of Transient Receptor Potential Vanilloid Type 1 by Gomisin A

Figure 2

The voltage-dependency and concentration dependency of gomisin A on capsaicin-stimulated inward current in oocytes expressing human TRPV1 receptor channel. (a) The representative current–voltage relationship was obtained using 1 sec duration voltage ramps from −100 to +80 mV, at a holding potential of −80 mV. Voltage steps were applied before and after the application of capsaicin in the presence or absence of gomisin A (100 μM). (b) The capsaicin-induced inward current in oocytes was elicited at a holding potential of −80 mV for the indicated time, in the presence of 1 μM capsaicin, after which the indicated concentrations of gomisin A were coapplied with capsaicin. Concentration response curves for the effect of gomisin A on oocytes expressing the TRPV1 receptor. The percentage inhibition by gomisin A was calculated based on the average of the peak inward current elicited by capsaicin and that of the peak inward current elicited by capsaicin plus gomisin A. Each point represents the mean ± SEM (/group).

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