Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP-β/c-Fos-MMP-7 Pathways

Figure 7

Schematic model of the proposed signaling pathways involved in suppressing cell migration and invasion by antcin-H in human RCC 786-0 cells. Antcin-H inhibits Src, FAK, and ERK1/2 phosphorylated activation, in turn decreasing paxillin, c-Fos, and C/EBP- activities, reducing the binding of c-Fos and phosphorylated-C/EBP- to AP-1 and C/EBP- response elements, thereby decreasing MMPs gene expression, especially MMP-7. Downregulation of MMP-7 and upregulation of TIMP-3 and TIMP-4 gene expression block the degradation of the extracellular matrix proteins and impair the cell invasion. Besides, reducing paxillin phosphorylation and vimentin expression prevents 786-0 cell motility.