Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP-β/c-Fos-MMP-7 Pathways

Figure 3

Suppression of wound-healing and disruption of lamellipodium formation by antcin-H. (a) Live cell time-lapse images at wound-healing front. 786-0 cells were cultured to 100% confluence on glass coverslips. After wound was made, fresh media without or with 100 M antcin-H were added. Cells were allowed to migrate; the time-lapse of live cell imaging was observed at 4 and 8 h. Scale bar, 20 m. (b) Immunostaining with FAK or (c) paxillin antibody. After 8 h wounding, the migrated cells were fixed, and the immunofluorescence staining was carried out using anti-phosphorylated-FAK and anti-phosphorylated-paxillin antibodies. The immunoreactive image was recorded by confocal microscope. Arrow, formation of lamellipodium. Scale bar, 20 m.

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