Uncommon Trimethoxylated Flavonol Obtained from Rubus rosaefolius Leaves and Its Antiproliferative Activity

Table 2

Antiproliferative activity of doxorubicin, hexane (HE), dichloromethane (DCM), and ethyl acetate (EA) fractions and compound 1 against human cancer cell lines.

Total growth inhibition (TGI) (µg/mL)
Cell lines	Doxorubicin	HE	DCM	EA	Compound  1
Glioma (U251)	4.1	35.4	51.1	>250	2.8
Breast (MCF-7)	0.62	45.2	17.8	>250	55.8
Multidrug-resistant ovary carcinoma (NCI-ADR/RES)	9.1	12.6	11.1	>250	18.8
Kidney (786-0)	0.46	30.7	19.3	>250	15.8
Lung, non-small cells (NCI-H460)	4.3	56.3	135.0	>250	14.1
Ovary (OVCAR-3)	2.4	46.1	89.7	>250	14.5
Colon (HT-29)	1.3	28.2	24.4	>250	>250
Leukemia (K-562)	>25	>250	250.0	>250	17.5
Nontumoral keratinocyte (HaCat)	0.79	10.4	25.8	>250	21.4

Assessed by the sulforhodamine B (SRB) assay. TGI values represent the concentration necessary (g/mL) for total inhibition of cancer cell proliferation. Values were determined through nonlinear regression analysis using the ORIGIN 8.0 (OriginLab Corporation). Dose range tested: 0.25 to 250 µg/mL. Experiment was conducted in triplicate.