Research Article

Anti-Inflammatory Activity of Triterpenes Isolated from Protium paniculatum Oil-Resins

Figure 4

Effect of the triterpene α,β-amyrone isolated from Protium ssp. on COX-2 expression in LPS-stimulated J774 cells. (a) J774 cells were pretreated with concentrations of 2.5, 5, and 10 μg/mL of α,β-amyrone and LPS (1 μg/mL) for 24 h. The cells were lysed, and the lysates were analyzed by immunoblotting with an anti-COX-2 antibody. The blot was stripped and reprobed with an anti-actin antibody to confirm equal loading. (b) Relative density of COX-2 protein was performed using the ImageJ Software. The significance was determined using ANOVA ( compared to LPS).
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