Research Article
Olive Leaf Extract Attenuates Obesity in High-Fat Diet-Fed Mice by Modulating the Expression of Molecules Involved in Adipogenesis and Thermogenesis
Table 2
Primer sequences and RT-PCR conditions.
| | Gene description | Primers | Sequences (5′→3′) | (°C) | Size (bp) |
| | Peroxisome proliferator-activated receptor (PPAR) | F | TTCGGAATCAGCTCTGTGGA | 55 | 148 | | R | CCATTGGGTCAGCTCTTGTG | | CCAAT/enhancer binding-protein α (C/EBP) | F | AAGGCCAAGAAGTCGGTGGA | 55 | 189 | | R | CCATAGTGGAAGCCTGATGC | | Leptin | F | CTCCAAGGTTGTCCAGGGTT | 55 | 143 | | R | AAAACTCCCCACAGAATGGG | | Cluster of differentiation 36 (CD36) | F | ATGACGTGGCAAAGAACAGC | 55 | 160 | | R | GAAGGCTCAAAGATGCCTCC | | Fatty acid synthase (FAS) | F | TTGCCCGAGTCAGAGAACC | 55 | 171 | | R | CGTCCACAATAGCTTCATAGC | | Wingless-type MMTV integration site family, member 10b (WNT10b) | F | TTTTGGCCACTCCTCTTCCT | 61 | 183 | | R | TCCTTTTCCAACCGAAAACC | | Secreted frizzled-related protein 5 (SFRP5) | F | CTGATGGCCTCATGGAACAG | 55 | 155 | | R | CTTGGTGTCCTTGCGCTTTA | | Galanin | F | GAGCCTTGATCCTGCACTGA | 60 | 121 | | R | AGTGGCTGACAGGGTCACAA | | Galanin receptor 1 (GalR1) | F | CCAAGGGGGTATCCCAGTAA | 55 | 147 | | R | GGCCAAACACTACCAGCGTA | | Galanin receptor 2 (GalR2) | F | ATAGTGGTGCTCATGCTGGAA | 60 | 134 | | R | AGGCTGGATCGAGGGTTCTA | | | | Protein kinase C (PKC) | F | CTGAGCGCTGCAAGAAGAAC | 60 | 146 | | R | TGGAAACTTTGATCCTGCACTGA | | Peroxisome proliferative activated receptor γ coactivator 1 (PGC-1) | F | TAAATCTGCGGGATGATGGA | 55 | 117 | | R | GTTTCGTTCGACCTGCGTAA | | Uncoupling protein 1 (UCP1) | F | GGTTTTGCACCACACTCCTG | 55 | 111 | | R | ACATGGACATCGCACAGCTT | | Cyclin D (cyc-D) | F | TGGGAAGTTTTGTTGGGTCA | 55 | 144 | | R | TCCTTGTCCAGGTAATGCCA | | E2F1 | F | CCTGGAGCATGTTAAAGAAG | 55 | 102 | | R | CCTCGAGACCAAAGTGATAG | | Sirtuin 1 (SIRT1) | F | AGCTCCTTGGAGACTGCGAT | 55 | 182 | | R | ATGAAGAGGTGTTGGTGGCA | | Cytochrome C oxidase subunit 2 (COX2) | F | CCGAGTCGTTCTGCCAATAG | 55 | 159 | | R | AACCCTGGTCGGTTTGATGT | | Mitochondrial transcription factor A (TFAM) | F | AGTGTGGCAGTCCATAGGCA | 55 | 123 | | R | CAGTGCTTTTAGCACGCTCC | | Nuclear respiratory factor-1 (NRF-1) | F | TTTCATGGACCCAGGCATTA | 55 | 119 | | R | TGGTGGCCTGAGTTTGTGTT | | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F | AGAACATCATCCCTGCATCC | 55 | 321 | | R | TCCACCACCCTGTTGCTGTA |
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