Research Article
Decaffeinated Green Coffee Bean Extract Attenuates Diet-Induced Obesity and Insulin Resistance in Mice
Table 1
Primer sequences and PCR conditions.
| Gene description | Primers | Sequences (5′-3′) | (°C) | Size (bp) |
| SFRP 5 | F | CTTGGTGTCCTTGCGCTTTA | 61 | 155 | R | CTGATGGCCTCATGGAACAG | DKK2 | F | TCATTCCCTGTTCTTCAGCG | 55 | 144 | R | GCATTTCCTTCAGATTGGCA | WNT10b | F | TTTTGGCCACTCCTCTTCCT | 61 | 183 | R | TCCTTTTCCAACCGAAAACC | Galanin | F | GAGCCTTGATCCTGCACTGA | 60 | 121 | R | AGTGGCTGACAGGGTCACAA | GalR1 | F | CCAAGGGGGTATCCCAGTAA | 60 | 147 | R | GGCCAAACACTACCAGCGTA | GalR2 | F | ATAGTGGTGCTCATGCTGGAA | 60 | 134 | R | AGGCTGGATCGAGGGTTCTA | PKC | F | CTGAGCGCTGCAAGAAGAAC | 60 | 146 | R | TGGAAACTTTGATCCTGCACTGA | Cyc-D | F | TGGGAAGTTTTGTTGGGTCA | 55 | 144 | R | TCCTTGTCCAGGTAATGCCA | PPAR2 | F | TTCGGAATCAGCTCTGTGGA | 55 | 148 | R | CCATTGGGTCAGCTCTTGTG | C/EBP | F | AAGGCCAAGAAGTCGGTGGA | 55 | 189 | R | CCATAGTGGAAGCCTGATGC | FAS | F | TTGCCCGAGTCAGAGAACC | 55 | 171 | R | CGTCCACAATAGCTTCATAGC | Leptin | F | CTCCAAGGTTGTCCAGGGTT | 55 | 143 | R | AAAACTCCCCACACAATGGG | CD36 | F | ATGACGTGGCAAAGAACAGC | 55 | 160 | R | GAAGGCTCAAAGATGCCTCC | aP2 | F | ACATGAAAGTGGGAGTG | 55 | 128 | R | AAGTACTCTCTGACCGGATG | TLR2 | F | TCTAAAGTCGATCCGCGACAT | 55 | 344 | R | TACCCAGCTCGCTCACTACGT | TLR4 | F | ACCTCTGCCTTCACTACAGA | 48.6 | 223 | R | AGGGACTTCTCAACCTTCTC | TNF | F | TGTCTCAGCCTCTTCTCATT | 55 | 156 | R | AGATGATCTGAGTGTGAGGG | MCP1 | F | CCAGCAAGATGATCCCAATG | 55 | 450 | R | CTTCTTGGGGTCAGCACAGA | IFN | F | ATGGCTAGGCTCTGTGCTTTCCT | 58 | 638 | R | GGGCTCTCCAGATTTCTGCTCTG | IFN | F | CCACAGCCCTCTCCATCAACTATAAGC | 56 | 372 | R | AGCTCTTCAACTGGAGAGCAGTTGAGG | IL-6 | F | TTGCCTTCTTGGGACTGATG | 55 | 162 | R | CCACGATTTCCCAGAGAACA | GAPDH | F | AGAACATCATCCCTGCATCC | 60 | 321 | R | TCCACCACCCTGTTGCTGTA |
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SFRP 5: secreted frizzled-related protein 5; DKK2: Dickkopf 2; WNT10b: wingless-type MMTV integration site family, member 10B; GalR1: galanin receptor 1; PKC: protein kinase C delta; Cyc-D: cyclin D; PPAR2: peroxisome proliferator-activated receptor gamma; C/EBP: CCAAT/enhancer-binding protein, alpha; FAS: fatty acid synthase; CD36: fatty acid translocase; aP2: adipocyte protein2; TLR: Toll-like receptor; TNF: tumor necrosis factor alpha; IFN: interferon; MCP1: monocyte chemoattractant protein 1; IL-6: interleukin-6; GAPDH: gyceraldehyde-3-phosphate dehydrogenase.
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