U-Bang-Haequi Tang: A Herbal Prescription that Prevents Acute Inflammation through Inhibition of NF-B-Mediated Inducible Nitric Oxide Synthase
Figure 6
Effect of UBT on LPS-inducible degradation of I-κBα and phosphorylation of mitogen-activated protein kinases (MAPKs). (a) The level of I-κBα and p-I-κBα in total lysates. Raw264.7 cells were stimulated with LPS or LPS + UBT for 30 min. Immunoblots are representative of results from repeated experiments. (b) Nuclear and cytosolic level of NF-κB. Lamin A/C and HSP 70 confirmed equal loading and purity of the each fraction. (c) The phosphorylation c-Jun NH2-terminal kinase (JNK), p38, and extracellular signal-regulated kinase (ERK) in total lysates. Raw264.7 cells were incubated with LPS UBT for 30 min. For A and C, data represents the mean ± S.E.M. from three separate experiments (significant as compared with control, ; significant as compared with LPS alone, ). JNK, c-Jun NH2-terminal kinase; ERK, extracellular signal-regulated kinase; MAPK, mitogen-activated protein kinase; UBT, U-bang-haequi tang.
