Molecular Mechanism of Yisui Shengxue Granule, a Complex Chinese Medicine, on Thalassemia Patients Suffering from Hemolysis and Anemia of Erythrocytes

Table 1

Primer used for detecting the gene expression by reverse real-time PCR.

Target gene	Primer sequence 5′-3′		Predicted amplification segments (bp)
	Forward primer	Reverse primer
β-Actin	GAG ACC TTC AAC ACC CCA GCC	AAT GTC ACG CAC GAT TTC CC	263
SPTA1	GAT CTT GAA GCC AAT GTC CA	CAA CTC CCT CCA CTG GTG	250
SPTB	AAC CGG GAT AAG GTC TTG AGT C	GGT GCT TTA GCC ATT TAT TGT GA	289
EPB4.1	TTA TCC ACT CAC TCA CCC TTC C	CTC ATC AGC AAT CTC GGT CTC C	369
BCL11A	CGC CAG AGG ATG ACG AT	CAG GCG TGG GGA TTA GA	138
α-Globin	CCC ACC ACC AAG ACC TAC TT	GCT CAC AGA AGC CAG GAA CTT	291
β-Globin	TAG CAA CCT CAA ACA GAC ACC A	ATC ACT AAA GGC ACC GAC CAC T	243
^Aγ-Globin	ATC AAT AAG CTC CTA GTC CAG ACG	CCA AAG CTG TCA AAG AAC CTC T	166
^Gγ-Globin	AGG AGG ACA AGG CTA CTA TCA CAA	CTT GAG ATC ATC CAG GTG CTT TAT	233
DNMT1	TAC CTG GAC GAC CCT GAC CTC	CGT TGG CAT CAA AGA TGG ACA	103
DNMT3a	TAT TGA TGA GCG CAC AAG AGA GC	GGG TGT TCC AGG GTA ACA TTG AG	111
DNMT3b	GGC AAG TTC TCC GAG GTC TCT G	TGG TAC ATG GCT TTT CGA TAG GA	132