Evidence-Based Complementary and Alternative Medicine

Research Article

The Predicted Proteomic Network Associated with the Antiarthritic Action of Qingfu Guanjieshu in Collagen-II-Induced Arthritis in Rats

Table 1

Identification of the differentially expressed proteins in the knee synovial tissue samples of the normal and vehicle-treated and QFGJS-treated CIA rats.


Spot no.		Swiss-Prot accession number	Theoretical Mr/pI	Sequence coverage (%)	Mowse scores	Number of unique peptides	Logarithm of average normalized volume			Fold change of average normalized volume between groups		Overall fold change of average normalized volume among three groups
Spot no.		Swiss-Prot accession number	Theoretical Mr/pI	Sequence coverage (%)	Mowse scores	Number of unique peptides	Normal	CIA	QFGJS	CIA/Normal	QFGJS/CIA	Max/Min^b	P value

1	IgG-2a Ig gamma-2A chain C region	P20760	35677/7.72	11	211	3	6.85	7.45	7.40	3.93	0.90	3.93
2	Vim vimentin	P31000	53757/5.06	30	282	12	6.34	6.60	6.91	1.82	2.04	3.73	0.011
3	Gstp1 glutathione S-transferase P	P04906	23652/6.89	20	268	3	6.24	6.68	6.55	2.70	0.75	2.70	0.006
4	Apolipoprotein C-III, isoform CRA_b	P06759	7847/4.65	36	163	2	6.03	6.33	5.90	2.00	0.37	2.69	0.011
5	Aldh6a1 methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial	Q02253	58396/8.44	7	90	3	6.46	6.89	6.56	2.71	0.47	2.71	0.019
6	Ca 3 carbonic anhydrase 3	P14141	29698/6.89	14	109	4	6.46	6.26	6.05	0.64	0.61	2.56	0.026
7	Vdac2 voltage-dependent anion-selective channel protein 2	P81155	32353/7.44	13	104	2	6.45	6.45	6.08	1.00	0.42	2.38	0.045
8	Tpi1 triosephosphate isomerase	P48500	27345/6.89	13	195	2	7.03	6.81	6.71	0.61	0.79	2.10
9	Ces3 carboxylesterase 3	P16303	62393/6.1	2	88	2	6.52	6.20	6.31	0.48	1.28	2.08	0.035
10	Des desmin	P48675	53481/5.21	7	176	3	7.04	6.73	6.79	0.49	1.15	2.06	0.02
11	RGD1560402 similar to phosphoglycerate kinase 1 (Pgk1)	IPI00372910	43604/6.15	10	97	3	6.63	6.71	6.39	1.20	0.48	2.08	0.034
12	Pgam1 phosphoglycerate mutase 1	P25113	28928/6.67	12	143	3	7.19	7.47	7.43	1.94	0.91	1.94	0.018
13	Anxa2 isoform short of Annexin A2	Q07936-1	38939/7.55	21	172	7	7.01	6.92	6.73	0.80	0.65	1.92	0.015
14	Gpx1 glutathione peroxidase 1	P04041	22472/7.66	16	121	3	6.61	6.88	6.73	1.90	0.71	1.90	0.033
15	Ivd isovaleryl-CoA dehydrogenase, mitochondrial	P12007	46862/8.03	14	112	5	6.86	6.71	6.59	0.70	0.76	1.86	0.002
16	Crp C-reactive protein	P48199	25737/4.89	21	193	4	6.48	6.63	6.75	1.41	1.31	1.85	0.048
17	Capzb F-actin-capping protein subunit beta	Q5XI32	30952/5.69	15	297	5	7.31	7.22	7.04	0.82	0.66	1.83	0.019
18	Idh3a isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial	Q99NA5	40044/6.47	15	199	5	7.01	6.89	6.75	0.77	0.73	1.79	0.027
19	RGD1565368 similar to glyceraldehyde-3-phosphate dehydrogenase	IPI00554039	36045/8.44	12	97	4	7.15	7.01	6.90	0.71	0.78	1.79	0.006
20	Selenbp1 selenium-binding protein 1	Q8VIF7	53069/6.1	16	149	7	7.18	6.99	6.94	0.65	0.90	1.71	0.008
21	Ogn osteoglycin	IPI00362931	34390/5.85	30	284	8	7.64	7.50	7.41	0.72	0.81	1.70	0.007
22	Ldhb L-lactate dehydrogenase B chain	P42123	36874/5.7	11	136	4	7.33	7.20	7.10	0.74	0.81	1.68	0.049
23	Actb actin, cytoplasmic 1	P60711	42052/5.29	13	209	4	7.13	6.99	6.91	0.74	0.83	1.63	0.023
24	Serum albumin	P02770	71244/5.82	9	120	5	6.80	6.73	6.60	0.85	0.73	1.61	0.004
25	S100a4 protein S100-A4	P05942	11997/5.04	52	233	6	7.32	7.53	7.50	1.60	0.95	1.60	0.038
26	Np purine nucleoside phosphorylase	P85973	32566/6.46	12	122	3	7.51	7.64	7.70	1.34	1.15	1.55	0.03
27	Capza2 F-actin-capping protein subunit alpha-2	Q3T1K5	33118/5.57	9	139	2	7.45	7.35	7.27	0.79	0.84	1.52	0.048
28	Mdh1 malate dehydrogenase, cytoplasmic	O88989	36631/6.16	12	73	4	7.27	7.17	7.09	0.80	0.83	1.51	0.024
29	Gsto1 glutathione S-transferase omega-1	Q9Z339	27936/6.25	12	146	3	6.86	6.80	6.70	0.88	0.78	1.46	0.05
30	Apoe apolipoprotein E	P02650	35788/5.23	33	251	11	7.15	7.31	7.24	1.45	0.85	1.45	0.015
31	Aldh2 protein	P11884	53791/5.83	12	396	6	7.35	7.20	7.21	0.71	1.03	1.42	0.045
32	Anxa1 Annexin A1	P07150	39147/6.97	16	316	6	7.38	7.28	7.23	0.79	0.89	1.41	0.033

The differently expressed proteins with fold change of Max/Min less than 1.4 after Progenesis SameSpots software analysis, repetitive proteins analyzed by MALDI-TOF-MS/MS, and unique peptides less than 2 with Mascot software were excluded.
Denote as the ratio of maximum (Max) and minimum (Min) average normalized spot volumes among the normal rats, vehicle-treated CIA, rats and QFGJS-treated CIA rats.