An Aqueous Extract of Radix Astragali, Angelica sinensis, and Panax notoginseng Is Effective in Preventing Diabetic Retinopathy
Figure 3
(a–d) Analysis of retinal vascular leakage in the retinas from (a) normal control rats (Control), (b) STZ-induced diabetic rats (Diabetic), and (c) STZ-induced diabetic rats treated with RRP (RRP) 3 weeks after the onset of diabetes using FITC-labeled albumin. Microscopic images of retinal flat mounts (a–c) showing fluorescently labeled albumin (arrowheads) in retinas of SD rats. (d) Quantification of the FITC-labeled albumin from (a), (b), and (c). Scale bars represent 100 μm. (e) Western blot analysis of retinal VEGF and occludin expression in Control, Diabetic, and RRP groups. (f) VEGF concentrations were measured in the retina of rats in Control, Diabetic, and RRP groups using an ELISA kit from R&D Systems. All data were expressed as mean ± SD (). versus normal SD rats; versus Diabetic SD rats.
