Potential Therapeutic Role of Hispidulin in Gastric Cancer through Induction of Apoptosis via NAG-1 Signaling

Figure 3

Effect of Hispidulin-induced G1/S phase arrest and changes expression of G1/S regulatory proteins in AGS cells. (a) The cell cycle analysis 6–48 h after Hispidulin treatment. (b) The Western blot analysis for p16, p21, cyclin D1, and cyclin E expression 6–48 h after treatment. Expression of 𝛽-actin was used as an internal control. (c) The western blot analysis for p16, p21, cyclin D1, and cyclin E expression in various dosage treatments. Expression of 𝛽-actin was used as an internal control. Each column represents the mean ± SD (^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001).

(a)

(b)

(c)