Reishi Protein LZ-8 Induces FOXP3+ Treg Expansion via a CD45-Dependent Signaling Pathway and Alleviates Acute Intestinal Inflammation in Mice
Figure 4
Adoptive transfer of LZ-8-stimulated CD4+ T cells alleviates DSS-induced colitis. Primary murine CD4+ T cells were cultured either without stimulation (naïve T), with rLZ-8 (LZ-8 T; 1 μg/mL), or with anti-CD3/28 antibodies (CD3/28 T; 1 μg/mL each) for 72 h. Colitis was induced by adding 4% DSS to the normal drinking water, with one group of mice receiving normal drinking water as a control (CTR). Intraperitoneal injections of PBS (200 μL/mouse), naïve T cells, LZ-8 T cells, or CD3/28 T cells (1.5 × 107 cells suspended in 200 μL PBS/mouse) were carried out 24 h prior to DSS induction. (a) The percentage of weight change in each mouse was monitored daily, and the data are presented as the mean ± SD. The asterisks indicate significant differences among DSS-treated groups (, ). (b, c) Colons from each mouse were excised for pathological (b) and histological (c) examination seven days after DSS induction. Representative specimens from five animals are presented. (c) The infiltrating leukocytes and intact goblet cells are highlighted by arrows and arrowheads, respectively.
