Spinal Serotonergic and Opioid Receptors Are Involved in Electroacupuncture-Induced Antinociception at Different Frequencies on ZuSanLi (ST 36) Acupoint

Table 1

Effects of EAc in different currents on the 1st, 3rd, and 7th days.

	Effect
Amp	Percentage change of pain threshold
	Min
	1st day
	0 min	15 min	30 min	60 min	90 min	120 min	150 min
Control	−2.34 ± 3.96	−4.91 ± 2.56	−0.89 ± 3.44	−2.32 ± 2.77	−1.62 ± 2.47	−0.41 ± 3.52	2.73 ± 3.50
2 Hz, 1 mA	39.33 ± 5.14	44.60 ± 3.78	51.41 ± 4.49	41.64 ± 4.62	30.12 ± 5.94	30.78 ± 5.21	23.46 ± 3.99
2 mA	25.03 ± 6.88	28.97 ± 7.08	23.65 ± 6.25	25.71 ± 6.56	22.22 ± 3.40	18.55 ± 2.47	20.53 ± 2.67
3 mA	32.76 ± 14.96	9.40 ± 10.64	3.63 ± 11.61	2.57 ± 10.90	4.67 ± 8.96	11.07 ± 8.76	13.23 ± 7.76
10 Hz, 1 mA	30.68 ± 8.00	26.25 ± 7.92	37.47 ± 8.16	26.71 ± 6.39	27.38 ± 6.31	22.34 ± 5.38	20.65 ± 4.03
2 mA	17.30 ± 4.13	25.90 ± 3.78	28.72 ± 9.06	28.51 ± 8.02	26.92 ± 5.16	7.02 ± 3.77	2.37 ± 1.50
3 mA	10.58 ± 17.05	27.77 ± 7.04	13.87 ± 10.54	17.95 ± 9.68	16.40 ± 9.96	16.69 ± 8.80	16.62 ± 7.54
100 Hz, 1 mA	31.34 ± 10.43	22.05 ± 7.83	25.47 ± 8.81	19.96 ± 6.92	19.88 ± 7.69	23.14 ± 11.83	25.30 ± 9.13
2 mA	25.93 ± 8.17	20.27 ± 6.31	21.29 ± 7.47	14.54 ± 5.18	12.20 ± 5.03	2.31 ± 1.88	1.34 ± 3.24
3 mA	10.59 ± 10.34	3.78 ± 15.37	2.43 ± 14.96	−5.91 ± 6.01	−6.49 ± 9.32	−9.36 ± 6.79	−0.25 ± 5.29
	3rd day
	0 min	15 min	30 min	60 min	90 min	120 min	150 min
Control	1.31 ± 2.74	1.09 ± 3.56	2.27 ± 3.63	0.03 ± 1.76	−1.42 ± 1.86	−0.96 ± 2.02	−0.97 ± 1.51
2 Hz, 1 mA	36.57 ± 5.46	28.70 ± 4.19	29.16 ± 6.49	25.19 ± 7.30	23.48 ± 6.10	19.30 ± 3.56	17.76 ± 4.54
2 mA	16.88 ± 4.01	17.66 ± 5.11	22.42 ± 3.86	11.78 ± 3.80	14.36 ± 3.62	16.53 ± 6.23	11.67 ± 7.02
3 mA	9.47 ± 8.53	4.01 ± 7.86	0.20 ± 9.56	−4.19 ± 6.64	−1.74 ± 5.01	2.98 ± 4.37	3.12 ± 4.07
10 Hz, 1 mA	32.53 ± 12.69	34.92 ± 6.99	61.73 ± 10.60	23.80 ± 5.49	27.27 ± 7.20	10.82 ± 3.75	12.41 ± 4.69
2 mA	22.21 ± 3.65	13.34 ± 5.82	16.51 ± 5.49	9.97 ± 5.26	4.32 ± 2.61	−0.87 ± 2.68	3.30 ± 4.53
3 mA	−5.36 ± 9.16	−3.85 ± 5.27	−5.38 ± 10.82	−9.10 ± 3.63	−6.81 ± 7.40	−2.76 ± 5.30	−2.73 ± 5.44
100 Hz, 1 mA	13.02 ± 9.21	28.95 ± 7.12	25.56 ± 7.96	19.35 ± 5.99	20.21 ± 7.46	16.44 ± 6.57	16.65 ± 6.40
2 mA	10.55 ± 3.38	16.18 ± 5.99	3.64 ± 3.01	5.43 ± 4.38	8.80 ± 3.51	−0.09 ± 3.60	0.69 ± 2.97
3 mA	18.32 ± 5.61	−2.27 ± 7.65	−5.62 ± 5.32	−0.30 ± 4.33	−0.13 ± 3.89	3.04 ± 3.90	3.63 ± 6.23
	7th day
	0 min	15 min	30 min	60 min	90 min	120 min	150 min
Control	1.28 ± 2.83	1.05 ± 3.74	2.18 ± 3.68	0.05 ± 2.08	−1.38 ± 1.78	−0.94 ± 2.05	−0.94 ± 1.82
2 Hz, 1 mA	35.71 ± 5.33	36.47 ± 3.80	34.76 ± 5.79	35.38 ± 3.75	28.84 ± 3.82	28.08 ± 2.51	26.89 ± 6.12
2 mA	17.47 ± 3.78	12.43 ± 3.22	17.28 ± 3.85	9.98 ± 4.22	8.86 ± 4.91	3.36 ± 2.37	3.08 ± 3.49
3 mA	−1.93 ± 7.94	−4.40 ± 5.65	−2.61 ± 4.41	4.41 ± 6.80	−0.98 ± 3.22	−4.44 ± 4.35	2.06 ± 3.76
10 Hz, 1 mA	36.09 ± 5.90	35.30 ± 4.77	29.37 ± 7.23	29.23 ± 7.24	14.14 ± 3.86	9.52 ± 4.66	10.60 ± 4.23
2 mA	12.49 ± 2.31	8.92 ± 5.82	4.26 ± 5.29	5.07 ± 4.85	2.39 ± 3.71	4.08 ± 5.07	1.20 ± 3.62
3 mA	2.59 ± 1.84	−0.12 ± 5.12	−2.53 ± 3.44	−1.40 ± 3.67	−5.97 ± 4.91	3.89 ± 2.66	7.47 ± 4.41
100 Hz, 1 mA	7.80 ± 3.07	22.35 ± 6.00	29.82 ± 8.99	10.14 ± 3.16	14.32 ± 4.23	9.31 ± 2.89	13.80 ± 3.51
2 mA	8.35 ± 3.43	2.59 ± 1.71	1.72 ± 1.92	5.73 ± 3.48	20.47 ± 3.73	−1.43 ± 2.55	−3.41 ± 2.10
3 mA	25.43 ± 10.43	3.44 ± 5.76	−15.95 ± 4.78	−11.89 ± 4.11	−9.48 ± 2.67	8.59 ± 4.74	6.81 ± 5.90

Data are shown as mean ± S.E.