Spinal Serotonergic and Opioid Receptors Are Involved in Electroacupuncture-Induced Antinociception at Different Frequencies on ZuSanLi (ST 36) Acupoint

Figure 6

Pretreatment with 5-HT antagonist influences EAc-induced antinociception in different frequencies on the 1st (a, b, c), 3rd (d, e, f), and 7th (g, h, i) days. (a), (d), (g): 1 mA, 2 Hz EAc; (b), (e), (h): 1 mA, 10 Hz EAc; (c), (f), (i): 1 mA, 100 Hz EAc, ↓: initiation point of EAc except sham group. ○: sham group; ⚫: ACSF (artificial CSF). ▲: PDB: (pindobind, 5-HT_1A antagonist, 0.5 μg/10 μL, i.t.). ■: KTS (ketanserin, 5-HT₂ antagonist 0.5 μg/10 μL, i.t.). ▼: LY-278584 (5-HT₃ antagonist, 0.5 μg/10 μL, i.t.). Data are shown as mean ± S.E. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 compared to the ACSF group (𝑛=10).

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