Apolar Bioactive Fraction of Melipona scutellaris Geopropolis on Streptococcus mutans Biofilm
Figure 1
Experimental scheme of biofilm treatments. (a) Biofilms were treated with HF or vehicle control (ethanol 12.5%) twice daily after the initial 24 h, and then at the 5th day, the biofilms were analyzed by inhibition of formation experiment and by scanning electron microscopy (SEM). (b) Biofilms were grown with no treatment until the 5th day, and then the biofilms were exposed to HF or vehicle according to the experimental protocols of time kill and pH drop assays.
