Isolation and Characterization of Adhesive Secretion from Cuvierian Tubules of Sea Cucumber Holothuria forskåli (Echinodermata: Holothuroidea)
Binding of adhesive protein from Cuvierian tubule extract to glass beads in the presence of various concentrations of urea. Glass beads (size, 2 mm) were incubated with adhesive protein extract in the presence of various concentrations of urea (1 M, 2 M, and 3 M; containing 0.5 M Tris-HCl, pH 7.5), as described in Materials and Methods. After washing, the glass beads were boiled in SDS sample buffer and the released protein was analyzed by 12% SDS PAGE. The gel was stained with Gel Code Blue Reagent. Relative band intensities corresponding to the adhesive protein were determined by scanning of the gel using an Odyssey Scanner and applying the Odyssey v.1.2 software to quantify the protein bands.
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