Homology-Driven Proteomics of Dinoflagellates with Unsequenced Genomes Using MALDI-TOF/TOF and Automated De Novo Sequencing
Figure 6
De novo sequencing and an MS-BLAST search validated a borderline hit produced using the MASCOT search. (a) The x- and y-axes show the mass-to-charge (m/z) ratio and the % abundance of the precursor ion fragments, respectively. The MS/MS spectrum was analyzed using DeNovo Explorer software to generate peptide (precursor 957.5391, 2480.3132, and 3237.6235) sequence candidates, (b) the table details ten peptide sequence candidates for the precursor 3237.6235 deduced from de novo sequencing, (c) the file corresponding to the spectrum in a2 and a3 and their de novo interpretation produced two candidate sequences with the quality score, and (d) and (e) the peptide sequence candidates from (b) and (c) were merged into an MS-BLAST query, and the search hit the same protein from A. tamarense. According to the MS-BLAST scoring scheme, the hits were confident.
