In Vitro Ultramorphological Assessment of Apoptosis on CEMss Induced by Linoleic Acid-Rich Fraction from Typhonium flagelliforme Tuber
Figure 6
Electromicrograph of untreated human T4 lymphoblastoid leukemia cells (CEMss) demonstrates the normal structure of CEMss cancer cell. The nucleus (N) contains evenly distributed chromatin and a large nucleolus (Nu), mitochondria (M) are large and numerous, rough endoplasmic reticulum (RER), ribosome (R) and the double membrane nuclear envelop (arrow) are also seen (×1000). In the treated cell nuclear chromatin marginisation (b-cm) (×1500), chromatin condensation (c-cc) (×1500) formation of apoptosis micronuclei (b-mn) characterize the apoptosis cell. The vacuolization (v) is also seen. Mitochondria of the control cell (a-m) shows more or less parallel cristae and of a treated cell (c-m) show vacuolization and loss of cristae. Cells were cultured in RPMI 1640 (25 ml flask) media maintained at 37°C and 5% CO2.
