Leaf Extracts of Calocedrus formosana (Florin) Induce G2/M Cell Cycle Arrest and Apoptosis in Human Bladder Cancer Cells

Table 1

Flow cytometry analysis in TCCSUP cells treated with Florin extracts.

Dose	Percentage (mean ± S.D.) of TCCSUP bladder cancer cells at each cell cycle phase after treatment with different concentrations of Florin extracts (μg/mL) at 24 h.
	subG1 (%)	G1 (%)	S (%)	G2/M (%)
0 (control)	0.8 ± 0.3	54.6 ± 0.5	15.2 ± 0.1	29.4 ± 0.3
6	1.4 ± 0.5	46.6 ± 2.5	16.0 ± 1.7	36.0 ± 2.7*
12	2.9 ± 0.1	24.9 ± 1.1	12.8 ± 0.8	59.4 ± 0.4**
25	1.3 ± 0.2	2.2 ± 0.2	1.9 ± 1.3	94.6 ± 1.1***

^aTCCSUP cell growth was arrested at the G2/M phase after Florin-extract treatment in a dose-dependent manner, and these cells were significantly different from the control cells.
^bCell numbers decreased significantly at the G1 phase (*𝑃<.05, **𝑃<.01, ***𝑃<.001).