Research Article

Leaf Extracts of Calocedrus formosana (Florin) Induce G2/M Cell Cycle Arrest and Apoptosis in Human Bladder Cancer Cells

Figure 6

Effect of treatment with Florin extracts on the amount of prometaphase microtubules in TCCSUP cells. (a) Cells were incubated with vehicle (0.1% dimethylsulfoxide) for 24 h. (b) Cells were treated with 25 μg/mL of Florin extracts, (c) 0.1 μg/mL of colchicine, or (d) 0.1 μg/mL Taxol for 24 h. Control indicates vehicle-treated cells. Microtubules (green) and nuclei (blue) are shown. Scale bar, 20 μm. Some of the β-tubulin are indicated by white arrows in (b), (c), and (d). (630x original magnification).
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