Purification and the Secondary Structure of Fucoidanase from Fusarium sp. LD8
Figure 3
Determination of the of the fucoidanase by SDS-PAGE. Picture of electrophoresis of fucoidanase: lane 1 is marker; lane 2 is the fucoidanase; (b) standard curve and function of the marker SDS-PAGE was conducted using discontinuous electrophoresis method with Phastsystem, 5.5% (w/v) polyacrylamide, pH 8.3, 250 V, 10 mA, in the concentration gel with 7.0% polyacrylamide (w/v), 250 V, 30 mA, in the separation gel. Protein was stained with Coomassie brilliant blue R-250. Molecular markers used were (220 kDa), -2 macroglobulin (170 kDa), -galactosidase (116 kDa), transferring (76 kDa), and glutamic dehydrogenase (53 kDa).
