The Efficacy of Dandelion Root Extract in Inducing Apoptosis in Drug-Resistant Human Melanoma Cells
Figure 8
Effect of DRE on G361 human melanoma cells. (a) Effect of DRE on G361 cell viability. G361 human melanoma cells were seeded on 96-well plates (~3000 cells/well) and treated at the indicated concentrations for 72 hours. The WST-1 dye was added to each well after every treatment period and incubated, as described in Section 2. Absorbances were read at 450 nm. (b) Induction of apoptosis in G361 cells by DRE. Typical apoptotic morphology was observed in G361 cells treated with DRE at 10 mg/mL concentrations for 72 hours. Cells were stained with Hoechst 33342 dye, before images were taken on a fluorescence microscope. Brightly stained, condensed bodies indicate apoptotic nuclei.
