The Efficacy of Dandelion Root Extract in Inducing Apoptosis in Drug-Resistant Human Melanoma Cells
Figure 7
Determining A375 cell revival after DRE-treatment. (a) Negatively stained A375 cell count after DRE-treatment. A375 cells were treated with DRE, at the indicated concentrations, for 72 hours, following which they were replated in fresh drug-media and incubated for 96 hours as described in Section 2. Negatively stained cells were collected and counted using Trypan blue. Statistical analysis was performed using the GraphPad Prism 5.0; *denotes a P-value <.05. (b) Continued apoptosis induction in A375 cells after DRE-treatment. A375 cells were treated with DRE at the indicated concentrations for 72 hours, as described in Section 2 After 96 hours of incubation in drug-free media, cells were stained with Hoechst 33342 dye and imaged on a fluorescence microscope.
