Research Article

The Efficacy of Dandelion Root Extract in Inducing Apoptosis in Drug-Resistant Human Melanoma Cells

Figure 2

Determining the toxicity of DRE on Normal Human Fibroblasts (NHFs) (a) Effect of DRE on NHF cell viability. NHFs were seeded on a 96-well plate (~2000 cells/well) and treated with DRE at the indicated concentrations for 96 hours. The WST-1 dye was added to each well after the 96-hour treatment period and incubated as described in Section 2. Absorbances were read at 450 nm. (b) Nontoxic effect of DRE on NHFs. NHFs were treated with DRE at the indicated concentrations for 96 hours. Cells were stained with Hoechst 33342 dye and imaged ona fluorescence microscope, as described in Section 2 (c) Effect of DRE on peripheral mononuclear blood cells (PBMCs). PBMCs were treated at the indicated concentrations for 48 hours before being stained with Hoechst 33342 dye and imaged on a fluorescence microscope. (d) Nontoxic effect of DRE on PBMCs. PBMCs treated in Figure 2(c) were quantified by manual counting of brightly stained apoptotic nuclei.
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