Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique

Figure 3

“Stretched” cell culture conditions induced a reorganization of the matrix. Slices of paraffin-embedded tissues produced with standard (a), with “flipped,” or with “stretched” cell culture conditions were subjected to staining using Masson’s trichrome staining. Matrix fibres were stained in blue whereas the cells were stained in purple/pink. The bar scale is 50 μm for all photographs. Pictures correspond to 3 representative tissues.

(a)

(b)

(c)