Loss of WNK1 Suppressed the Malignant Behaviors of Hepatocellular Carcinoma Cells by Promoting Autophagy and Activating AMPK Pathway

Figure 5

Knockdown of WNK1 suppressed the malignant behaviors of HCC cells by suppressing autophagy and inactivating AMPK pathway. (a, b) si-AMPK transfection reduced the expression patterns of AMPK, Beclin 1, LC3 II/I, and increased P62 level, and the above results were reversed after si-WNK1 treatment. (c, d) si-AMPK transfection increased the OD values of Huh7 and Hep3B cells, while si-WNK1 treatment counteracted the effect of si-AMPK transfection on the OD values of Huh7 and Hep3B cells. (e–g) si-AMPK transfection increased the clone number of Huh7 and Hep3B cells, while si-WNK1 treatment counteracted the effect of si-AMPK transfection on the clone number of Huh7 and Hep3B cells. , vs. control, ^## vs. si-WNK1, ^&, ^&& vs. si-AMPK.

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